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Professor Zihe Rao

Picornavirus uncoating intermediate captured in atomic detail

Ren J, Wang X, Hu Z, Gao Q, Sun Y, Li X, Porta C, Walter TS, Gilbert RJ, Zhao Y, Axford D, Williams M, McAuley K, Rowlands DJ, Yin W, Wang J, Stuart DI, Rao Z, Fry EE.

It remains largely mysterious how the genomes of non-enveloped eukaryotic viruses are transferred across a membrane into the host cell. Picornaviruses are simple models for such viruses, and initiate this uncoating process through particle expansion, which reveals channels through which internal capsid proteins and the viral genome presumably exit the particle, although this has not been clearly seen until now. Here we present the atomic structure of an uncoating intermediate for the major human picornavirus pathogen CAV16, which reveals VP1 partly extruded from the capsid, poised to embed in the host membrane. Together with previous low-resolution results, we are able to propose a detailed hypothesis for the ordered egress of the internal proteins, using two distinct sets of channels through the capsid, and suggest a structural link to the condensed RNA within the particle, which may be involved in triggering RNA release. [ Nat Commun. 2013;4:1929. PMID: 23728514 ] [ PDF ]

Crimean-Congo hemorrhagic fever virus nucleoprotein reveals endonuclease activity in bunyaviruses

A sensor-adaptor mechanism for enterovirus uncoating from structures of EV71

Guo Y, Wang W, Ji W, Deng M, Sun Y, Zhou H, Yang C, Deng F, Wang H, Hu Z, Lou Z* & Rao Z*. 2012. Crimean–Congo hemorrhagic fever virus nucleoprotein reveals endonuclease activity in bunyaviruses. Proc Natl Acad Sci USA,109(13):5046-51. [PMID: 22421137][download PDF]

Wang X, Peng W, Ren J, Hu Z, Xu J, Lou Z, Li X, Yin W, Shen X, Porta C, Walter TS, Evans G, Axford D, Owen R, Rowlands DJ, Wang J*, Stuart DI*, Fry EE*, Rao Z*. & 2012. A sensor-adaptor mechanism for enterovirus uncoating from structures of EV71. Nat Struct Mol Biol, 19(4): 424–429. [PMID: 22388738][download PDF]

Crystal structure of the mitochondrial respiratory membrane protein Complex II

Design of wide spectrum inhibitors targeting the coronavirus main protease (Mpro)

Crystal structure of the polymerase PA(C)-PB1(N) complex & PA(N) from an avian influenza H5N1 virus

Sun F, Huo X, Zhai YJ, Wang AJ, Xu JX, Su D, Bartlam M & Rao Z*. 2005. Crystal Structure of Mitochondrial Respiratory Membrane Protein Complex II. Cell, 121: 1043-1057. [download PDF]

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Yang H, Xie WQ, Xue XY, Yang KL, Ma J, Liang WX, Zhao Q, Zhou Z, Pei DQ, J. Ziebuhr, R. Hilgenfeld, K. Y. Yuen, Wong L., Gao GX, Chen SJ, Chen Z, Ma DW, Bartlam M & Rao Z*. 2005. Design of Wide-Spectrum Inhibitors Targeting Coronavirus Main Proteases. PLoS Biology, 3(10):e324, 1742-1752. [download PDF]

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He X, Zhou J, Bartlam M, Zhang R, Ma J, Lou Z, Li X, Li J, Joachimiak A., Zeng Z, Ge R, Rao Z* & Liu YF*. & 2008. Crystal structure of the polymerase PA(C)-PB1(N) complex from an avian influenza H5N1 virus. Nature, 454(7208):1123-6. [download PDF]

ST news (www.stdaily.com) [download PDF]

 

Tsinghua University news [download PDF]

"Crowning achievement": Nature Research Highlights [download PDF]

 

"Common viruses have Achilles heel": New Scientist [download PDF]

 

"Casting a wide net to fight coronaviruses": PLoS Biology public release [download PDF]

 

"Casting a wide net to fight coronaviruses": PLoS Biology synopsis [download PDF]

"Yuan P, Bartlam M, Lou Z, Chen S, Zhou J, He X, Lv Z, Ge R, Li X, Deng T, Fodor E, Rao Z* & Liu Y*. 2009. Crystal structure of an avian influenza polymerase PA(N) reveals an endonuclease active site. Nature, 458(7240):909-13 [download PDF]  

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